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Oil Accumulation by the Oleaginous Diatom Fistulifera solaris as Revealed by the Genome and Transcriptome

01/29/2015
The Plant Cell

Type

Article dans des revues

Authors

Tanaka Tsuyoshi
Maeda Yoshiaki
Veluchamy Alaguraj
Tanaka Michihiro
Abida Heni
Maréchal Eric
Bowler Chris
Muto Masaki
Sunaga Yoshihiko
Tanaka Masayoshi
Yoshino Tomoko
Taniguchi Takeaki
Fukuda Yorikane
Nemoto Michiko
Matsumoto Mitsufumi
Wong Pui Shan
Aburatani Sachiyo
Fujibuchi Wataru

Oleaginous photosynthetic organisms such as microalgae are promising sources for biofuel production through the generation of carbon-neutral sustainable energy. However, the metabolic mechanisms driving high-rate lipid production in these oleaginous organisms remain unclear, thus impeding efforts to improve productivity through genetic modifications. We analyzed the genome and transcriptome of the oleaginous diatom Fistulifera solaris JPCC DA0580. Next-generation sequencing technology provided evidence of an allodiploid genome structure, suggesting unorthodox molecular evolutionary and genetic regulatory systems for reinforcing metabolic efficiencies. Although major metabolic pathways were shared with nonoleaginous diatoms, transcriptome analysis revealed unique expression patterns, such as concomitant upregulation of fatty acid/triacylglycerol biosynthesis and fatty acid degradation (β-oxidation) in concert with ATP production. This peculiar pattern of gene expression may account for the simultaneous growth and oil accumulation phenotype and may inspire novel biofuel production technology based on this oleaginous microalga.